Functional involvement of carbonic anhydrase in calcium transport of the chick chorioallantoic membrane

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Calcium-transport function of the chick embryonic chorioallantoic membrane. II. Functional involvement of calcium-binding protein, Ca2+-ATPase and carbonic anhydrase.

This study aimed to investigate the mechanism of active calcium transport in the chick embryonic chorioallantoic membrane (CAM) by assessing the functional involvement of three previously identified, putative components of the transport pathway. These components are a calcium-binding protein (CaBP), Ca2+-activated ATPase and carbonic anhydrase. Using specific reagents, including antibodies and ...

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Calcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization.

During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included...

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Sodium transport and electrical properties of the chick chorioallantoic membrane.

Transport and electrical properties of the chick chorioallantoic membrane (CAM) were studied in order to find the osmoregulatory organ which helps to compensate the renal filtration-reabsorption disbalance of chick embryos. It could be shown that CAM resembles Na+ transporting epithelia in that active Na+ absorption is responsible for the potential difference and short circuit current, which co...

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Transepithelial calcium transport in the chick chorioallantoic membrane. I. Isolation and characterization of chorionic ectoderm cells.

The chicken eggshell supplies approximately 80% of the calcium found in the hatchling chick. The mobilization of eggshell calcium into the developing embryo involves the transepithelial transport of large amounts of calcium in a development-specific manner. The cells responsible for the transport of eggshell calcium into the embryonic circulation are the ectodermal cells of the chorioallantoic ...

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Supplemented eggshell restores calcium transport in chorioallantoic membrane of cultured shell-less chick embryos.

It was previously reported (Tuan, 1980a) that the development-specific expression of calcium transport and related functions in the chick embryonic chorioallantoic membrane (CAM) requires the continuous presence of the eggshell, the calcium source of the embryo. To further understand the mechanism of action of the eggshell on the CAM functions, this study reports the effects of eggshell supplem...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1978

ISSN: 0264-6021

DOI: 10.1042/bj1760067